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Samples were taken to monitor growth of a cell culture, and data entered into a table shown below.
The log of the cell concentration was already solved for in the last column.
Time after inoculation (min.) Cells/mL
0
60
120
180
log(N)-log(No)
t
1.2e8
= K
9.2e8
4.6e9
1.1e10
Log of Cell Concentration
T: time
•K: Slope/Growth Constant
N: Number of Cell at time point
.NO: Starting number of cells
8.07
8.96
The following equation allows for you to solve for the growth constant of any cell culture using the
number of cells at the starting and final time points along with the amount of time the culture was
growing.
9.66
10.04
2. Use the above equation to solve for the slope/growth constant K from the time points t=0 to
t-60. Answer to four decimal places and show your work.
Sagot :
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