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What would be the consequence of treating the vector, before ligation, with calf intestinal phosphatase?
A. It would prevent the plasmid's DNA from reversing polarity during ligation.
B. It would prevent the ends of the plasmids from being ligated.
C. It would ligate the ends of the plasmids.
D. It would reverse the polarity of plasmids' DNA
Sagot :
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