ENZYME CATALYSIS LABORATORY REPORT SHEET ACTIVITY 1: ENZYME CONCENTRATION 1. Construct a hypothesis addressing the effect concentration will have on rate of reaction. 2. What chemical reaction is being catalyzed in the experiment? Label the substrate(s), enzyme, and product(s). 0% 25% 50% 75% 100% Depth of H2O2 Solution 2.1 cm (d) 2.1 cm 2.1 cm 2.1 cm 2.1 cm Trial 1 Time 180 sec 84.61 sec 43.52 sec 36.90sec 25.90 sec Trial 2 Time 180 sec 92.25 sec 38.16 sec 34.36 sec 23.57sec Trial 3 Time 32.53 sec 18.82 sec Average Time (t) 180 sec 88.43 sec 40.84 Sec 34.5 sec 22.76 sec Rate of the Reaction (R = d/t) 0.012 cm/sec 0.02 cm/sec 0.051 cm/sec 0.061 cm/sec 0.0923cm/sec 4. Using the computer, graph rate of reaction on the y-axis and percent concentration of enzyme on the x-axis. If the points are linear, draw a "best-fit" straight line through or near all the data points. 0.1 0.09 0.08 8 0.07 0.06 Rate of Reaction cm/sec 0.05 0.04 0.03 0.02 0.01 0 0% 20% 100% 120% 40% 60% 80% Concentration of Enzyne 5. Based on the information in the data table and your graph, explain the relationship between percent concentration of catalase and rate of reaction. 6. Did your actual results match your hypotheses? If not, why? 7. Using the graph, you created, predict the rate of the reaction if catalase concentrations are 10%, 60% and 85%. This will be done by extrapolating from the graph. In order to do this, take each catalase concentration above and locate it on the x-axis. From that point, draw a vertical line until it intersects the "best fit" line. From that point of intersection, draw a horizontal line to the y-axis. Where this intersection occurs would be the approximate rate of reaction.