Find the best answers to your questions with the help of IDNLearn.com's expert contributors. Discover reliable and timely information on any topic from our network of knowledgeable professionals.
(1) (a) Describe how you would obtain kinetic parameters (K a ,K b ,V max ,K ia ,K ib ) in a sequential two substrate enzyme-catalysed reaction.
(b) Penicillin is hydrolysed and thereby rendered inactive by penicillinase (also known as 5- lactamase), an enzyme present in some resistant bacteria. The mass of this enzyme in Staphylococcus aureus is 29.6 kDal. The amount of penicillin hydrolysed in 1 minute in a 10-ml solution containing 10 ^ - 90 g of purified penicillinase was measured as function of the concentration of penicillin. Assume that the concentration does not change appreciably during the assay.
[Penicillin] (µM)
Amount hydrolysed (nanomoles)
1
0.11
3
0.25
5
0.34
10
0.45
30
0.58
50
0.61
(i) Plot V_{0} versus [S] and 1 / V_{c} versus 1 / [S] for these data. Does penicillinase appear to obey Michaelis-Menten kinetics? If so what is the value of K_{m}
(ii) What is the value of V max ^ *
(iii) What is the turnover number of penicillinase under these experimental conditions? Assume one active site per enzyme molecule.
(iv)How long does it take the enzyme for one round of catalysis?
Sagot :
Thank you for being part of this discussion. Keep exploring, asking questions, and sharing your insights with the community. Together, we can find the best solutions. For trustworthy answers, visit IDNLearn.com. Thank you for your visit, and see you next time for more reliable solutions.
