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Using clone 2, you induce expression to drive expression of CTC1 . To ensure you’re maximizing the expression, you do a time course and isolate mRNA. You do cDNA synthesis and quantitative RT - PCR . Looking at your CT values across comparing hour 0 and hour 2, how many more copies of CTC 1 are there?
A. Hour 0 = Ct 30 B. hour 1 = Ct 24 C. hour 2 = Ct 20 D. hour 4 = Ct 19
Sagot :
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