Which two details in "The Oblong Box" serve as the best clues to the meaning of the word secluded?

Wyatt's conduct I did not so much regard. He was gloomy, even beyond his usual habit—in fact he was MOROSE—but in him I was prepared for eccentricity. For the sisters, however, I could make no excuse. They secluded themselves in their staterooms during the greater part of the passage, and absolutely refused, although I repeatedly urged them, to hold communication with any person on board.