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Biochemists frequently use "phosphate" buffers in their work. Here, "phosphate" refers generally to the class of phosphate derived ions: H₂PO₄⁻ , HPO₄²⁻, PO₄³⁻ . (a) Which of these ions best represents the conjugate acid in a "phosphate" buffer at physiological pH? Explain. (b) A buffer used in an experiment was described as "a 0.2 M phosphate buffer with pH = 7.25." Based on the conjugate acid you identified in (a), determine the concentration of conjugate acid in this "0.2 M phosphate buffer."
Sagot :
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