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A student needs to determine the concentration of Tryptophan (Try), which absorbs UV light at 282 nm. The absorbance of Tryptophan in an unknown analyte sample previously diluted 1:8 was 0.673 at 282 nm. Calibration of the UV-Vis spectrometer was performed by serial dilution of a Tryptophan standard (230 ppm). The student diluted the standard in the following ratios: 1:10, 1:20, 1:50, 1:60, and 1:70, and determined absorbances of 1.923, 0.942, 0.443, 0.367, and 0.294 respectively. What is the concentration of Tryptophan in the unknown analyte? Determine the calibration equation and the correlation coefficient value.
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