Answered

Experience the convenience of getting your questions answered at IDNLearn.com. Join our Q&A platform to access reliable and detailed answers from experts in various fields.

A student wants to use western blotting to confirm the presence of human Sonic hedgehog (Shh) in samples run on SDS-PAGE and transfered to a nitrocellulose membrane. The antibody reagent box in their lab contains Mouse anti-Shh MAb and Chicken anti-mouse IgG-HRP. What substrate will they need in order to perform colorimetric detection of Shh on their western blot

Sagot :

Ogorwyneidn

Answer:

BCIP/NBT

Explanation:

BCIP/NBT is a colorogenic substrate used for AP. AP stands for alkaline phosphatase. When they are catalyzed with this alkaline, we would have a product that has a dark blue or probably a purple color. NBT stands for nitro blue tetrazolium. Both of these work as substrate for the reaction. BCIP oxidizes BCIP to form this color.

We appreciate every question and answer you provide. Keep engaging and finding the best solutions. This community is the perfect place to learn and grow together. Your search for answers ends at IDNLearn.com. Thank you for visiting, and we hope to assist you again soon.