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what are the three limitations of an ELISA

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Queensierra005idn

Answer:

Enzyme-linked Immunosorbent Assay, also know as ELISA are used to determine if an antibody is present in a patient's blood sample. They are found in bodily fluids.

Explanation:

The three important limitations are:

1) A positive result confirming a presence of an antibody but it not necessarily making the patient sick

2) A false negative where the amount of antibodies is too low to be measured

3) Another positive result may occur if an unrelated antibody reacts with the antigen non specifically.

Ajinemsidn

The most common limitations of ELISA is based on the antibodies that are being detected..

Enzyme-linked immunosorbent assay (ELISA) is a advanced laboratory test procedure which is used to detect proteins known as antibodies. These antibodies are proteins that are generated in immune system response to antigens of invading microorganisms.

There are some limitations or restrictions seems while performing ELISA test includes:

  • A false positive result may occur if an unrelated antibody reacts with the antigen non-specifically.
  • When there are false positives in persons that has recovered from a disease. This is because the body can continue to produce antibodies even when the person had recovered.
  • When there is false negatives. This occurs when patients may be poor producers of an antibody or may have some interfering substances in their blood.

Learn more here:

https://brainly.com/question/22704116

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