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Sentence Arrange - Put the steps of DNA replication in order by writing a number in the space before each statement. _____Two new molecules of DNA are created. _____DNA polymerase attach the free-floating nucleotides to the exposed nitrogen bases. _____ Helicase begins to break the hydrogen bonds between nitrogen bases. _____DNA is stabilize by the enzyme topoimerase. _____DNA ligase mends Okazaki fragments.

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Answer:

1. Helicase begins to break the hydrogen bonds between nitrogen bases.  

2. DNA is stabilized by the enzyme topoisomerase.

3.DNA polymerase attaches the free-floating nucleotides to the exposed nitrogen bases.  

4. DNA ligase mends Okazaki fragments.

5. Two new molecules of DNA are created.  

Explanation:

Replication is a cellular mechanism by which a DNA molecule is used as template to synthesize two identical DNA molecules, and then each daughter cell receives one DNA molecule during cell division. First, DNA unwinds at dynamic structures called replication forks: a helicase enzyme opens up the double helix of the DNA at the replication fork by breaking hydrogen bonds that hold complementary DNA strands together. Subsequently, a topoisomerase enzyme removes positive supercoils generated in front of the replication fork. In the next step, a DNA polymerase extends primers (i.e., short RNA sequences that serve as templates for the starting point of replication) by adding nucleotides to the 3' end of the growing strand. The replication continues in the 5' to 3' direction on the leading strand, while the strand of the DNA that runs 5' to 3' away from the replication fork (known as the lagging strand) must be synthesized in fragments called Okazaki fragments. Subsequently, a DNA ligase enzyme facilitates the joining of the Okazaki fragments by forming phosphodiester bonds between nucleotides, thereby resulting in a continuous lagging strand. Finally, replication ends when converging replication forks meet.

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