How to get Live Imaging of the Cellular Genome via CRISPR System?

The spatiotemporal organization of chromatin structure plays a critical role in regulating lineage-specific gene expression during cellular differentiation and embryonic development, via contributing to the functional output of the genome, which can be dynamically amplified or suppressed. However, there is little known about how the genomes being modified and how their structural organization regulating function output.

Question

01-06-2021
Biology

Answered

Explore a diverse range of topics and get answers from knowledgeable individuals on IDNLearn.com. Ask any question and receive accurate, in-depth responses from our dedicated team of experts.

How to get Live Imaging of the Cellular Genome via CRISPR System?

The spatiotemporal organization of chromatin structure plays a critical role in regulating lineage-specific gene expression during cellular differentiation and embryonic development, via contributing to the functional output of the genome, which can be dynamically amplified or suppressed. However, there is little known about how the genomes being modified and how their structural organization regulating function output.

Sagot :

Your presence in our community is highly appreciated. Keep sharing your insights and solutions. Together, we can build a rich and valuable knowledge resource for everyone. Your questions deserve precise answers. Thank you for visiting IDNLearn.com, and see you again soon for more helpful information.

What Is The Width Of A Rectangle Prism With The Length Of 13 Feet Volume Of 11,232 Ft.³ And Height Of 36

Upton Sinclair, Frank Norris, And Ida Tarbell Made Their Greatest Contributions To The Progressive Movement By (1) Working To End Political Corruption

How Did Trade Routes Influence The Renaissance Beginning In Italy?

1.Salts Consist Of Atomic Particles That Have An (blank) Charge. fill In The Blank 2. The Metallic Atoms Have A Positive Charge, While The Nonmetallic Atoms Hav

Determine The Number Of Ways To Choose A Set Of 9 Pencils From A Selection Of 10.

What Is The Molar Mass Of Sulfur Hexafluoride Gas If The Density Is 22.4g/L At STP?

Which Explains What Earth's Climate Would Be Like If The Amount Of Greenhouse Gases In The Atmosphere Was Increased? a. The Gases Would Absorb More Heat Energy

The Ratio Of Men To Women Working For A Company Is 8 To 5 . If There Are 80 Women Working For The Company, What Is The Total Number Of Employees?

Are Species In The Same Family More Or Less Closely Related Than Species In The Same Class?

Tim Makes Wooden Salad Bowls To Sell Monthly At A Craft Fair. Each Month He Spends $45 On Speciality Wood. At The Fair Tim Sells All The Bowls For $22 Including

Luisvaschettoidn Luisvaschettoidn · Answer 1 · 2021-06-05T20:05:00-04:00

Answer:

a catalytically inactive Cas9 enzyme (dCas9) is fused to fluorescent labels (e.g., GFP)

Explanation:

The CRISPR-Cas9 genome editing system consists of two parts: 1- a Caspase enzyme called 'Cas9' with catalytic activity that cuts DNA strands by hydrolyzing phosphodiester bonds, and 2-a single guide RNA (sgRNA) that directs Cas9 to target sites with complementarity to nucleotides of the sgRNA in order to edit these sequences. Using a catalytically dead Cas9 (dCas9) it is possible to repurpose the CRISPR-Cas9 system, for example, by fusing dCas9 to epigenetic enzymes for epigenome editing or by fusing dCas9 to the Green Fluorescent Protein (GFP) for imaging of the cellular genome. Thus, it is possible to use dCas9 to create a customizable DNA labeler that can be complemented with a fluorescence microscope and thus track multiple genomic loci in living cells.

Sagot :

Other Questions