How will you isolate DNA from a pure fungal culture

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05-07-2021
Biology

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How will you isolate DNA from a pure fungal culture

Sagot :

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Adeoladokun31idn Adeoladokun31idn · Answer 1 · 2021-07-09T20:23:40-04:00

Answer:

Explanation:

There are several ways of isolating DNA from a pure fungal culture but you can use the CTAB method.

In this method, genomic DNA can be extracted.

First of all, the cell walls of fungal mycelia can first be broken down by grinding it using glass rods or you can use or liquid nitrogen. Place it in a plate. You can now CTAB extraction buffer , then incubate at 65°C, then let it undergo purification with phenol:chloroform:isoamyl alcohol 25: 24: 1 and carry out precipitation with isopropanol . Then the DNA can be extracted and then dissolved in 50 μl of pure water.

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