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Genetic engineering involves various stages.
1 human DNA is inserted into bacterial plasmid DNA 2 recombinant plasmid inserted into bacteria 3 restriction enzyme cuts bacterial plasmid DNA 4 restriction enzyme cuts human DNA
What is the correct sequence for genetic engineering?
FIRST: cDNA is synthesized to be used as a gene of interest: processed (spliced) mRNA (no introns) is reverse transcribed to cDNA using reverse transcriptase, an enzyme from retroviruses
SECOND: Restriction enzyme used to cut GOI
THIRD: Same Restriction enzyme used to cut plasmid (sticky ends are created)
FOURTH: GOI connects/hybridizes with plasmid due to base-pairing
FIFTH: DNA ligase connects the recombinant DNA strands
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