Jeslinroseidn
Answered

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Substrate: N-benzoyl-L-tyrosine ethyl ester (BTEE) 0.001M in 50% aqueous
her
methanol.
Buffer:0.1M tris-HCl, pH 7.8, containing 0.1M CaCl₂.
Enzyme:1mg/ml chymotrypsin A in 0.001M HCI.
N.B. The enzyme provided is a stock solution which should be diluted 50-fold with
0.001M HCl immediately before use.

How do I do this calculation abs the final volume should be 5ml

Sagot :

Mickymike92idn

0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.

What is dilution?

Dilution is a process of making a solution of lower concentration from a solution of higher concentration by the addition of solvent to a given volume of the solution of higher concentration.

Dilution of solutions is done using the dilution formula in order to determine the given volume of diluent or stock solution required. The dilution formula is given below:

  • C1V1 = C2V2

where:

  • C1 = Initial concentration of enzyme
  • C2 = Final concentration of enzyme
  • V1 = Initial volume
  • V2 = Final volume

For the enzyme dilution;

C1 = 1 mg/mL

C2 = 1/50 mg/ml = 0.02 mg/ml

V= ?

V2 = 5 ml

V1 = C2V2/C1

V1 =  0.02 * 5/1 = 0.1 mL

Therefore, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl in order to prepare a 50-fold diluted enzyme.

Learn more about dilution at: https://brainly.com/question/24881505

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