Jeslinroseidn
Answered

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The enzyme provided is a stock solution which should be diluted 50-fold with 0.001M HCl immediately before use.

Enzyme :1mg/ml chymotrypsin A in 0.001M HCl



How do I work out this calculation, if I want the 5ml of the diluted enzyme?

Sagot :

Mickymike92idn

In order to prepare a 50-fold diluted enzyme, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl.

What is dilution?

Dilution is a process whereby a solution of lower concentration is prepared from one of a higher concentration.

Dilution is done by using the dilution formula below:

  • C1V1 = C2V2

C1; Initial concentration of enzyme = 1 mg/mL

C2; Final concentration of enzyme = 1/50 mg/ml = 0.02 mg/ml

V1; Initial volume of enzyme = y

V2; Final volume = 5.0 ml

V1 = C2V2/C1

V1 =  0.02 * 5/1 = 0.1 mL

Therefore, in order to prepare a 50-fold diluted enzyme, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl.

Learn more about dilution at: https://brainly.com/question/24881505

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