Jeslinroseidn
Answered

IDNLearn.com connects you with experts who provide accurate and reliable answers. Join our knowledgeable community to find the answers you need for any topic or issue.

The enzyme provided is a stock solution which should be diluted 50-fold with 0.001M HCl immediately before use.

Enzyme :1mg/ml chymotrypsin A in 0.001M HCl



How do I work out this calculation, if I want the 5ml of the diluted enzyme?

Sagot :

Mickymike92idn

In order to prepare a 50-fold diluted enzyme, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl.

What is dilution?

Dilution is a process whereby a solution of lower concentration is prepared from one of a higher concentration.

Dilution is done by using the dilution formula below:

  • C1V1 = C2V2

C1; Initial concentration of enzyme = 1 mg/mL

C2; Final concentration of enzyme = 1/50 mg/ml = 0.02 mg/ml

V1; Initial volume of enzyme = y

V2; Final volume = 5.0 ml

V1 = C2V2/C1

V1 =  0.02 * 5/1 = 0.1 mL

Therefore, in order to prepare a 50-fold diluted enzyme, 0.1 mL of the stock solution of the enzyme is taken and made up to 5.0 mL with 0.001M HCl.

Learn more about dilution at: https://brainly.com/question/24881505

#SPJ1

Thank you for joining our conversation. Don't hesitate to return anytime to find answers to your questions. Let's continue sharing knowledge and experiences! IDNLearn.com is dedicated to providing accurate answers. Thank you for visiting, and see you next time for more solutions.