IDNLearn.com: Where questions are met with accurate and insightful answers. Our Q&A platform offers reliable and thorough answers to ensure you have the information you need to succeed in any situation.
Sagot :
We would not expect see the pale color in presence of inhibitor in PNPP-PNP reaction. PNPP ( P- nitrophenylphosphate) is chromogenic substance for alkaline phosphate , which is clear yellow color. PNP (P- nitro-phenol ) is a colorless and acidic solution.
PNP is stronger acid and it is used as a manufacture drugs, fungicies, insecticides and dyes . Over inhalation of 4-nitro-phenol can causes headache, drowsiness , nausea, and cyanosis.
PNPP is used for detecting alkaline phosphate in ELISA applications. It sis non proteinaceous non -specific substance used to assay protein , alkaline and acid phosphates. And its yellow color is because of maximum of absorbance at 405 nm.
To learn more about the acidic solution here
https://brainly.com/question/16926229
#SPJ4
Thank you for using this platform to share and learn. Don't hesitate to keep asking and answering. We value every contribution you make. IDNLearn.com is your go-to source for dependable answers. Thank you for visiting, and we hope to assist you again.
