What would happen if in a pcr reaction you used a dna polymerase isolated not from thermus aquaticus, but from an organism that grows at lower temperature?.

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03-08-2022
Biology

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What would happen if in a pcr reaction you used a dna polymerase isolated not from thermus aquaticus, but from an organism that grows at lower temperature?.

Sagot :

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Tutorconsortium31idn Tutorconsortium31idn · Answer 1 · 2022-08-06T14:26:49-04:00

There will be little to no DNA

Taq DNA polymerase is the most common enzyme used for PCR amplification. This enzyme is extremely heat resistant with a half-life of 40 minutes at 95°C. At its optimal temperature (72°C), nucleotides are incorporated at a rate of 2–4 kilobases per minute.

What is PCR reaction ?

In PCR, a segment of the genome to be amplified is chosen using short synthetic DNA fragments called primers. Multiple rounds of DNA synthesis are then used to amplify that segment

A specific target DNA sequence can be exponentially amplified using the Polymerase Chain Reaction (PCR), which enables the isolation, sequencing, or cloning of a single sequence among many.

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What is PCR reaction ?

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