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In the malate–aspartate shuttle, electrons are transferred from oxaloacetate to form malate.
A crucial mechanism employed by mitochondria, the malate-aspartate shuttle system, also known as the malate shuttle, enables electrons to pass through the impermeable membrane separating the cytosol as well as the mitochondrial matrix. Glycolysis produces the electrons which are required for oxidative phosphorylation.
The liver, heart, as well as kidney, include the malate-aspartate shuttle, which produces about three molecules of ATP for every cytosolic NADH molecule. Under aerobic conditions, it constitutes quantitatively the most significant shuttle for such reoxidation of cytosolic NADH in vertebrate tissues.
Therefore, In the malate–aspartate shuttle, electrons are transferred from oxaloacetate to form malate.
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