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Increasing the salinity of the environment a little at a time until the ATP usage reaches a minimum additional investigation can be used to determine when the cells are in an isotonic solution.
Isotonic solutions are those that have the same amount of water and other solutes in them as the cytoplasm of a cell. Since there is no net gain or loss of water, placing cells in an isotonic solution will not cause them to either shrink or swell.
Cytoplasm- A viscous liquid termed cytoplasm, which is contained within the cell membrane, fills each cell. Water, salts, and proteins make up the majority of it. Eukaryotic cells are entirely enclosed within their cytoplasm, both inside and out.
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