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Sagot :
DNA is being denature with high temperature application. DNA's hydrogen bonds are disrupted by heat. The DNA flexibility is being boosted by heat thereby resulting to a condense folding of DNA chain (double-stranded). Due to this effect of destabilizing the DNA, the DNA duplex is entirely detached. Although DNA replication also requires heating, high temperatures (melting point) will greatly impact its replication. DNA extracted will give increase yield at lower temperature than in high temperature. Enzymes involved in DNA replication are affected by temperature. It can be denatured at high temperature and its actions will slow down at low temperature.
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