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To introduce unnatural amino acids into proteins, a better strategy is to inject unnatural amino acids into cells using tRNA or aminoacyl-tRNA synthetase pairs.
The incorporation of unnatural amino acids (UNAAs) into protein design has become a central discipline in synthetic biology. The 20 standard amino acids (SAAs) alone can no longer meet the growing demands of basic and applied science due to their limited functional chemistry. Replacing SAA with UNAA could provide proteins with new physicochemical properties and biological functions. In addition, advances in cell-free protein synthesis (CFPS) systems have paved the way for the precise and efficient incorporation of UNAA into proteins.
UNAA is incorporated into proteins by altering unnatural biological mechanisms including codons, tRNAs, aminoacyl-tRNA synthetase (aaRS), elongation factor (EF), retention factor (RF), and ribosomes.
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