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A 3-carbon dicarboxylic acid called malonate is linked to Coenzyme A in malonyl CoA. Using the biotin cofactor of the enzyme acetyl-CoA carboxylase, malonate is created from acetyl-CoA by adding CO₂ .
What is the source of malonyl-CoA?
Acetyl-CoA carboxylase (Acc1p) produces malonyl-CoA, which is produced as the first and only step in the production of fatty acids. The activity of Acc1p is closely controlled both transcriptionally and post-translationally, and Snf1p protein kinase phosphorylation of Acc1p inhibits its activity.
Is malonyl-CoA an extender or starter?
All forms of PKSs use malonyl-CoA as an extender unit. Malonyl-CoA is always incorporated into the developing polyketide through a decarboxylative Claisen condensation between the carboxyl group of a thioester and the enolate ion produced by malonyl-decarboxylation. CoA's .
Is CO2 used in fatty acid synthesis?
Although CO2 or HCO3 are not absorbed into the fatty acids, the need for carbon dioxide as a bicarbonate ion is a crucial component in the production of fatty acids. This indicates a method where CO2 is initially given to a precursor, serving its role, and then eventually withdrawn.
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