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Sagot :
Before staining, bacterial smears are "heat fixed" on microscope slides to adhere the cells to the slide and prevent them from washing off during the staining process. Fixation is the next phase, and it's often carried out using a Bunsen burner's flame. Consequently, the right response is (b).
One must first make the bacterial smears on the slide before staining the bacterial specimen for microscopy. Transferring a liquid solution of the bacteria onto the slide, drying the smear, and finally slightly heating the smear to securely bind it to the slide are the three main phases in this process.The preparation of a smear is the first step in the majority of bacterial staining processes. Cells from a culture are applied in a thin layer to a tiny region of a microscope slide, dried, and then fixed to the slide using heat or other chemical fixatives. This procedure is known as a smear preparation.
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