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Cas9 cleaves both strands of the target DNA. Which of the following could occur when cells will try to repair the damage after cleavage by Cas9? (Select all that apply.)
mutations that result in changes to the open reading frame (ORF) of the targeted gene
loss-of-function mutations within the targeted gene
the insertion of a DNA sequence into the targeted gene
no change in the targeted gene
deletions of the targeted gene

Sagot :

The following options are all valid - Modifications to the targeted gene's open reading frame (ORF) as a result of mutations

- Mutations that cause loss-of-function in the targeted gene

- A DNA sequence is inserted into the desired gene.

- The targeted gene has not changed

- Gene deletions related to the target

A frameshift mutation is a genetic change brought on by an insertion or deletion that changes how the DNA sequence is read.

A frameshift variation happens when nucleotides are added or removed, shifting the grouping and altering the coding for all amino acids downstream. Typically, the protein that results is not useful.

A frameshift mutation results in a complete modification of a protein's amino acid sequence. Because ribosomes read the mRNA strand in terms of codons, or groups of three nucleotides, this modification takes place during translation. The reading frame is a name for these categories.

Learn more about mutation is visit here;

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