researchers added dinitrophenol (dnp) to cultured myocyte mitochondria. if dnp allows facilitated diffusion of protons across the inner mitochondrial membrane without passage through the atp synthase, which change to atp levels is expected over time? a. increasing atp levels after addition of dnp b. decreasing atp levels after addition of dnp c. constant atp levels after addition of dnp d. constant atp levels before and after addition of dnp

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06-12-2022
Biology

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researchers added dinitrophenol (dnp) to cultured myocyte mitochondria. if dnp allows facilitated diffusion of protons across the inner mitochondrial membrane without passage through the atp synthase, which change to atp levels is expected over time? a. increasing atp levels after addition of dnp b. decreasing atp levels after addition of dnp c. constant atp levels after addition of dnp d. constant atp levels before and after addition of dnp

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Qwbeigeidn Qwbeigeidn · Answer 1 · 2022-12-09T00:46:13-05:00

Expected ATP change over time with DNP is option B: decreasing ATP levels after addition of DNP.

With the addition of DNP, protons would be able to freely permeate across the membrane, quickly shutting down ATP synthase. Answer C is not a viable option because as DNP starts to work, some protons may still pass through the synthase molecule and any ADP molecules that are already loaded would be converted to ATP. Nevertheless, it cannot be said that zero ATP will be formed immediately after the addition of DNP.

DNP is an uncoupler, it disrupts the ATP synthesis by leaking protons across the inner mitochondrial membrane. As a result of stopping the pumping of hydrogen ions, the proton gradient cannot occur across the membrane. As a result, the inner mitochondrial membrane's pH will drop, and ATP production will be less effective.

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