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All of the resulting bacteria contain the pUC18 plasmid without the human gene.
In E. coli, the pUC18 plasmid cloning vector is often employed. Double-stranded circular DNA makes up the molecule (2686 base pairs in length). Large DNA segments may be successfully cloned thanks to pUC18's tiny size. In the right host strains, the pUC18 plasmid induces complement deficiencies in -galactosidase as well as ampicillin resistance.
The MCS region in pUC18 is the opposite of that in pUC19. Both orientations of the restriction enzyme-generated DNA fragments toward the lac promoter can be cloned using pUC18 and pUC19.
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