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sputum sample on sarabaud-dextrose plates. Differentiate the prospective pathogens from the "normal" occupants of the upper respiratory tract, and focus on the potential pathogens, according to growth on the plate.
Instead of using a sample that is primarily made up of saliva, sputum should be taken from the lower respiratory tract. sputum sample need to be taken as soon as the sun rises. There are numerous techniques to analyse pathogens a sputum sample: The sediment from the unfixed specimen can be centrifuged and then inspected directly on a wet mount. To grab the entire specimen, reach under the patient's lower lip. Tell the patient to refrain respiratory from putting any secretions from their nose or mouth into the vial that contains the medium.
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