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The master mix now contains: 0.16 mL of 10 μM ethanol, 0.2 ml of 10 μM NAD+, and 0.54 mL buffer. We will add 0.1 mL of 1 mg/mL ADH so that the total volume will be 1 mL. What is the substrate concentration in this master mix? a) 0.016 mM b) 1.6 mM c) 0.16 mM d) 0.0016 mM
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