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The master mix now contains:
0.16 mL of 10 μM ethanol, 0.2 ml of 10 μM NAD+, and 0.54 mL buffer. We will add 0.1 mL
of 1 mg/mL ADH so that the total volume will be 1 mL. What is the substrate
concentration in this master mix?
a) 0.016 mM
b) 1.6 mM
c) 0.16 mM
d) 0.0016 mM

The master mix now contains:
0.16 mL of 10 μM ethanol, 0.2 ml of 10 μM NAD+, and 0.54 mL buffer. We will add 0.1 mL
of 1 mg/mL ADH so that the total volume will be 1 mL. What is the substrate
concentration in this master mix?
a) 0.016 mM
b) 1.6 mM
c) 0.16 mM
d) 0.0016 mM

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