Jeslinroseidn
Answered

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Substrate: N-benzoyl-L-tyrosine ethyl ester (BTEE) 0.001M
Buffer:0.1M tris-HCl, pH 7.8, containing 0.1M CaCl₂.
Enzyme:1mg/ml chymotrypsin A in 0.001M HCI.
N.B. The enzyme provided is a stock solution which should be diluted 50-fold with
0.001M HCl immediately before use.

How do I do this fold dilution calculation ?

Sagot :

Mickymike92idn

Based on the dilution formula, 0.1 mL of the stock solution of the enzyme is required to prepare a 50-fold diluted enzyme in 0.01 M HCl.

How can 50-fold dilution of the enzyme be done?

The 50-fold dilution of the stock enzyme solution can be done by using the dilution formula to determine the given volume of the stock solution required.

The dilution formula is given below:

  • C1V1 = C2V2

where:

  • C1 = Initial concentration of enzyme
  • C2 = Final concentration of enzyme
  • V1 = Initial volume
  • V2 = Final volume

From the given data for the enzyme dilution;

C1 = 1

C2 = 1/50 = 0.02

V1 = x

V2 = 5 ml

Making V1 subject of formula in the dilution formula:

V1 = C2V2/C1

V1 =  0.02 * 5/1 = 0.1 mL

Therefore, 0.1 mL of the stock solution of the enzyme is required to prepare a 50-fold diluted enzyme in 0.01 M HCl.

Learn more about dilution at: https://brainly.com/question/24709069

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